Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiforum A.DC.

NATALIA URBAŃSKA, JADWIGA NARTOWSKA,AGNIESZKA SKORUPSKA, DARIUSZ RUSZKOWSKI, JOANNA GIEBUŁTOWICZ, OLGA OLSZOWSKA

Abstract

The sum of saponins in the hairy root lines (6, 17) of Platycodon grandiforum A.DC. was compared. Hairy root line 6 showed a higher total saponin content (6.92%) than the line 17 (6.01%). According to the Chinese Pharmacopoeia standards the content of saponins in Platycodi Radix should be not less than 2%. Our results seem to indicate that the hairy root culture of Platycodon grandiforum A.DC. is a good source of saponins. The Haemolytic Index of the hairy root line 6 was 1600. Digitonin was used as a reference. Moreover, the haemolytic activity of TLC subfractions of saponins varied.
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