Evaluation of the analytical method for a chromatographic profile assessment of the essential oil obtained from Pimpinella saxifraga s.l.

ANNA GRYS, EWA KOMOROWSKA, SEBASTIAN MIELCAREK, ZDZISŁAW ŁOWICKI, KAROL LATOWSKI, WALDEMAR BUCHWALD

Abstract

Pimpinella saxifraga s.l. has been used for medicinal purposes for a long time. The root is a part of medicinal properties composed by essential oils in about 0.4%. The composition of these essential root oils consists of about sixty chemicals. The aim of the current experiment was assessing the analytical method of chromatographic profile utilizing essential oil of Pimpinella saxifraga. This essential oil was isolated with use of Deryng apparatus according to Polish Pharmacopoeia. The method for assessing the chromatographic profile of the essential oil was split-up gas chromatographic capillary column. The quantitative composition of the essential oil was calculated with use of method of normalization. The identity and the compositions of the essential oil from Pimpinella saxifraga roots was analyzed on Clarus 500 gas chromatograph equipped with capillary columns with flame ionization detector (FID), automatic dispenser and computer with Total Chrom Navigator software Perkin Elmer. Chromatographic system was characterized by range of retention time, area peaks, tailing factor and compounds’ separation. This method of volatile oil substance marking was validated following the ICH standards, by its precision, accuracy and linearity.
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