SRC kinase mRNA transcription changes in testosterone-induced rat ventral prostate lobes under the influence of Epilobium angustifolium extract
Abstract
The aim of this study was to investigate the influence of standardized crude aqueous
Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of SRC kinase
mRNA - a representatives of non-genomics xenobiotics signaling pathway in prostate
ventral lobes of testosterone-induced, castrated rats. We have shown that in all analyzed
groups induced by testosterone an elevation of SRC kinase mRNA transcription was observed,
in comparison to control animals (not receiving the testosterone), (p<0.05). Finasteride
in rats induced by testosterone caused the strongest inhibition of SRC mRNA
transcription (p<0.05). In rats receiving testosterone and the plant extract a ca. 90% decrease
of mRNA level was observed vs. testosterone-induced animals (p<0.05), while in
testosterone-induced animals receiving concomitantly E. angustifolium extract and finasteride
the observed reduction reached 87.3% (p<0.05).
We did not observed, however, any positive feedback between studied plant extract and
finasteride in the inhibitory activity (p<0.05). Further experimental studies should be performed
in order to the understanding the molecular basis of interactions, the efficacy and
safety of tested plant extract.