Chemical composition, antioxidant and antimicrobial activity of Nepeta persica Boiss. essential oil

MOHADDESEH MAHBOUBI, NASTARAN KAZEMPOUR, FATEMEH GHAZIAN, MOHSEN TAGHIZADEH

Abstract

The essential oil from aerial parts of Nepeta persica Boiss was obtained by steam distillation and was analyzed by GC and GC/MS. Fourteen components were identified as accounting for 97.3% of total oil composition. The 4aα,7α,7aα-nepetalactone (80%) and Spiro[5.6]dodecane (14.2%) were the main components of essential oil. Antimicrobial activity of essential oil against different kinds of microorganisms was determined by micro-broth dilution assay. The minimal inhibitory concentration and minimal lethal concentration values of oil were in the ranges from 1-8 to 1-16 μl/ml, respectively. Most of all, the oil was sensitive to Candida albicans, Staphylococcus aureus and Klebsiella pneumoniae. Antioxidant activity was evaluated by 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The IC50 values of N. persica essential oil and BHT were nearly 0.03%, 2.98%, respectively and in β-carotene/linoleic acid system, the essential oil did not show effective antioxidant activity.
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