Screening of various solvents and extracts of Achillea latiloba Ledeb. ex Nordm for antibacterial and antifungal activities
In this study the antibacterial and antifungal activities of extracted Achillea latiloba Ledeb. ex Nordm (Asteraceae) samples in acetone, ethyl acetate, ethanol, methanol and dimethyl sulfoxide (DMSO) from Trabzon Province (Turkey) were investigated. Antimicrobial activity of A. latiloba varied depending on the extract of samples, dosage of extracts, and the extraction solvents for all test microorganisms. Staphylococcus aureus, Streptococcus salivarius, Klebsiella pneumoniae, Escherichia coli, Salmonella enteritidis, Streptococcus pneumoniae, Bacillus cereus, Listeria monocytogenes, Streptococcus mutans, Pseudomonas aeruginosa, Bacillus licheniformis, Micrococcus luteus, Bacillus subtillis, Proteus vulgaris and Candida albicans were studied with use of disc diffusion and agar dilution method. The results indicated that each of the crude extracts of Achillea latiloba exhibited a more or less pronounced antibacterial and antifungal potency both in Gram-positive and Gram-negative bacteria and fungi. While in the Gram-negative group, the most sensitive microorganism to Achillea latiloba were S. enteritidis and Streptococcus mutans which is Gram-positive. In the Gram-positive group, the microorganisms most sensitive to Achillea latiloba were Streptococcus mutans and L. monocytogenes. However, the least sensitive microorganism was P. vulgaris. The results presented in this paper suggest that Achillea latiloba possesses additional antimicrobial activities that has an effect against some Gram-negative, Gram-positive bacteria and fungi.