Electrophoretic identification of fucoidan in Fucus vesiculosus L.



Macroalgae have been found to be built of a number of compounds including polysaccharides, proteins, lipids, water and a variety of macro- and microelements. Brown algae contain fucoidan, a fucose-containing sulfonated polysaccharide. Fucoidan from brown algae such as the common bladder wrack (Fucus vesiculosus L.) is now widely examined in many countries for its interesting biological and therapeutic properties. Its antivirus, antitumor and cardioprotective effects have already been proved. In this study, fucoidan was separated and identified in bladder wrack thalli during extraction in water and hydrochloric acid. Preliminary tests allowed for identifying fucoidan during five-minute extraction. A simple, repeatable analytic procedure was developed using inexpensive apparatus for cellulose acetate membrane electrophoresis.
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