Influence of cadmium on protein profile of flax varieties (Linum usitatissimum L.)

MILENA SZALATA, TOMASZ WRÓBEL, ALEKSANDRA LUWAŃSKA, MARLENA SZALATA, KAROLINA WIELGUS

Abstract

The aim of the study was the evaluation of the influence of cadmium on protein profile of flax varieties (Linum usitatissimum L.). Linola and Norlin explants were cultured on control medium Dorothy and with addition of 25 and 75 mg/l Cd(NO3)2. Extracts were separated on DEAE-Cellulose (20 mM Tris-HCl buffer, 0.2-1 M NaCl). Protein content was evaluated by measuring the absorbance at wavelengths 280 and 254 nm. Linola was characterized by proteins occurrence in fractions eluted by 0.4 and 0.6 M NaCl at 25 mg/l of Cd(NO3)2, at 75 mg/l and additionally by 0.3 M NaCl. Norlin showed response in the form of proteins appeared at 0.2, 0.4, 0.5 and 0.6 M NaCl gradient at 25 mg/l of cadmium, at higher concentration in fractions eluted by 0.2, 0.5 and 0.6 M NaCl. Electrophoretic analysis showed an increase in the protein bands intensity above 60 kDa and under 52 kDa in extracts from flax cultivated with Cd(NO3)2. Studies showed appearance of new proteins during stress condition.
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