Establishment of Rhodiola kirilowii hairy roots using Agrobacterium rhizogenes LBA 9402

MONIKA ZYCH, AGNIESZKA PIETROSIUK, MONIKA KARASIEWICZ, ANNA BOGACZ, RADOSŁAW KUJAWSKI, PRZEMYSŁAW M. MROZIKIEWICZ, ANNA KRAJEWSKA-PATAN, MIROSŁAWA FURMANOWA

Abstract

For the first time hairy roots of the Rhodiola genus were obtained by infection of the of Rhodiola kirilowii plantlets with Agrobacterium rhizogenes LBA 9402. First transgenic roots appeared 11 days after infection. the transformation process was confirmed by the poly- merase chain reaction (Pcr). dNA analysis showed that rol B gene and aux 1 gene from ri plasmid of Agrobacterium rhizogenes were incorporated into the plant genome of Rhodiola kirilowii.
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