Enhanced isoflavones accumulation in methyl jasmonate-treated in vitro cultures of kudzu (Pueraria lobata Ohwi)

BARBARA THIEM, ALDONA KRAWCZYK

Abstract

Kudzu, Pueraria lobata (Willd.) Ohwi (Fabaceae) is a woody climber plant that naturally grows in tropical and subtropical East Asia, where the species is highly valued for its medicinal properties. Methyl jasmonate (MJ) stimulated isoflavones accumulation in Pueraria lobata whole plant cultures in vitro after 7 days of elicitation. Isoflavonoid content increased by the addition of 50, 100 and 200 μM of MJ. However, the plantlets growth was inhibited by increasing of MJ concentration. The elicitor (100 μM) enhanced total isoflavones production in plantlets of P. lobata to 10.44 mg/g dry wt. Moreover, MJ stimulated accumulation of daidzein and genistein. It was found that the total content of isoflavones increased in comparison with that found in the control plantlets, while content of genistein was 4–fold higher than control plants but the content of daidzein increased from undetectable level up to 0.54 mg.g-1 dry mass. The study shows that the content of active compounds in whole plant cultures of P. lobata may be enhanced by elicitation with MJ.
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