Identification and determination of extracellular saccharase in cell suspension of Chelidonium majus L.

K. MIČIETA, J. STANO, M. KOREŇOVÁ, V. BLANÁRIKOVÁ, M. FULMEKOVÁ, P. MATEJKA

Abstract

A simple and rapid procedure for identification and determination of extracellular saccharase is described, using a culture medium of Chelidonium majus L. (celandine) cell suspension cultures. Sucrose was used as a substrate for determination of the extracellular and intracellular activities of the studied enzyme. The culture medium (without cells) was used for identification and determination of extracellular enzyme activity. Intracellular activity was estimated using cell suspension.
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