Methods to promote germination of Kelussia odoratissima Mozaff., an Iranian endemic medicinal plant
The response of seeds germination from Apiaceae, Kelussia odoratissima, which have interesting horticultural and medicinal properties, was established. In order to optimize germination factors for the first time, seeds were exposed to different types of treatment. In the first experiment, different times of stratification (0, 15, 30 and 45 days at 4˚C) were tested, and the best stratification time was 45 days. In the second experiment, the results of the first experiment were followed by various leaching durations (0, 12 and 24 hours with running tap water), Gibberellic Acid (GA3) (0, 500 and 1000 ppm concentrations for 12 hours) and germination temperatures (5, 10 and 15˚C). The treatments, arranged as a factorial experiment, involved three replications per treatment in a completely randomized design. Results revealed that long stratification (45 days) stimulated germination percentage (GP) and mean germination time (MGT) compared to lower times of stratification. It seems that applying external GA3 has no effect on K. odoratissima seed germination. Different temperatures during seed germination affect MGT and GP significantly as the highest MGT and GP were at 5˚C and 10˚C, respectively. The duration of leaching diminished GP and the best MGT was observed in treatments with no application of leaching. In conclusion, the best germination of K. odoratissima was achieved at 10˚C after 45 days stratification in 4˚C.