α-Galactosidase in immobilized cells of Citrulus vulgaris L.

J. STANO, K. MIČIETA, E. TOKHTAEVA, M. FULMEKOVÁ

Abstract

Cells of suspension culture Citrullus vulgaris cv. "Samara" were permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest alfa-galactosidase activity was at pH 5.4 and 60°C. The hydrolysis of substrate was linear for 3.5 h reaching 65-70% conversion of the substrate. The cells characterized with high enzyme activity, and stability in long-term storage showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts).
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