Callus tissues of Rhodiola Kirilowii (Regel) Maxim. – dynamic of growth and active compounds production

ANNA KRAJEWSKA-PATAN, MARIOLA DREGER, WALDEMAR BUCHWALD, MAŁGORZATA GÓRSKA-PAUKSZTA, SEBASTIAN MIELCAREK, MAREK BARANIAK, ALINA MŚCISZ, MIROSŁAWA FURMANOWA, PRZEMYSŁAW M. MROZIKIEWICZ

Abstract

Rhodiola Kirilowii (Regel) Maxim. (Crassulaceae) is a traditional medicinal plant used in North Asia and China, especially in the cardiopulmonary disorders in the hypoxic conditions induced by high altitude. The presented results are the part of the investigations carried out in the Branch of Medicinal Plants of the Institute of Natural Fibres and Medicinal Plants in cooperation with the Department of Biology and Pharmaceutical Botany, Medical University in Warsaw on R. Kirilowii plants and tissue cultures. The aim of recent study was to determine the growth dynamics and active compounds production during the cultivation of callus tissues of R. Kirilowii on solid/liquid media. Tissue cultures of R. Kirilowii shown the ability to produce all the active compounds determined in the roots of plants of Polish origin. It is worth emphasizing, that rosavins, according to known literature, were not detected in roots of plants growing in Asia. The best time for collection the tissues from solid medium was fifth or sixth week of the culture – the tissues were growing dynamically and the contents of the main active compounds was high. The material from suspension should be collected in 12–15 days after inoculation. The obtained results will be applied in future investigations on the use of R. Kirilowii extracts in the experimental hypoxia in rats. Key words: Rhodiola Kirilowii, roots of plant, in vitro cultivation, callus tissues, suspension cultures, hypoxia, salidroside, rosavins, phenolic acids, tannins, growth dynamics
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