Micropropagation of Harpagophytum procumbens (Burch.) DC. ex Meisn.; the effect of cytokinins on shoot multiplication

RENATA GRĄBKOWSKA, HALINA WYSOKIŃSKA

Abstract

The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 μM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 μM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 μM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months. Key words: cytokinins, gibberellic acid, Harpagophytum procumbens, micropropagation
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