The changes in the proteins profile induced by cadmium stress in flax (Linum usitatissimum L.)

MILENA SZALATA, MARLENA SZALATA, KAROLINA WIELGUS

Abstract

Influence of Cd(NO3)2 concentration on the process of flax (Linum usitatissimum L.) germination and changes occurring on the level of synthesized proteins or peptides were evaluated. The studies did not reveal differences in seed germination in control conditions and in presence of cadmium solution. Extracts obtained from two-week-old seedlings were exposed to ammonium sulphate saturation (0–30 and 50–80%). Ion exchange chromatography on DEAE Cellulose revealed appearance of proteins or peptides rich in the cysteine residues which were not present in control group (absorbance 280 and 254 nm). Particular proteins were observed in three extract fractions eluted by NaCl gradient (0.3–0.5 M NaCl) what could suggests formation of (Cys-rich) protein or peptides in presence of the cadmium solution during detoxification process. The largest absorbance indicated a high concentration of (Cys-rich) proteins or peptides related probably to incubation with cadmium solution observed at extract fraction eluted with 0.4 M NaCl. Key words: Flax, heavy metals, soil contamination, phytoremediation
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