Determination of 10-deacetylbaccatine III in Taxus baccata needles by micellar electrokinetic chromatography

MARCIN ZAREK, PIOTR WALIGÓRSKI

Abstract

The optimization of the analytical buffer composition for micellar electrokinetic chromatography in order to make simple separations of 10-deacetylbaccatine III from sample matrices (other taxanes and other compounds) and to determine its concentration in the samples was performed. The samples were prepared from crude Taxus baccata L. needle extracts. The best results were obtained with the buffer containing 25 mmol/dm3 Tris-HCl (pH 9.0), 40 mmol/dm3 sodium dodecyl sulphate and 15% acetonitrile for a wavelength 230 nm (Diode Array Detector). Finally determination of 10-DAB III concentration in T. baccata needles collected from four different populations of Southern Poland was performed (mean concentration respectively: Cisy w Nowej Wsi – 0.135 mg/g d.w., Cisowa Góra – 0.185 mg/g d.w., Zadni Gaj – 0.143 mg/g d.w., Cisy nad Liswartą – 0.150 mg/g d.w.). There were no significant differences in the mean concentration of 10-deacetylbaccatine III among samples from different populations. Key words: 10-deacetylbaccatine III; 10-DAB III; Taxus baccata; common yew, MEKC; micellar electrokinetic chromatography; taxanes
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