Seed protein and esterase isozyme profile among the accession of lemongrass [Cymbopogon flexuosus (Nees ex Steud.) Wats] collected from environmentally different sites

ANIRUDDHA SARMA, HEMEN SARMA, GAUTAM K. HANDIQUE, TARUN CH. SARMA, ARUN K. HANDIQUE

Abstract

Seed protein profile and esterase isoenzme were studied in ten accessions of lemongrass (Cymbopogon flexuosus) collected from northeastern India belonging to two major chemotypes – citral and geraniol rich essential oil. Total four esterase isozyme bands were observed with Rm values in the range of 0.394 to 0.798. Among them one with Rm value 0.747 was unique as it was consistently present in all the accessions irrespective of citral or geraniol rich chemotype. For seed protein, SDS-PAGE analysis of seven accessions (RLJ-TC-1, RLJ-TC-4, RLJ-TC-5, RLJ-TC-8, RLJ-TC-9, RLJ-TC-10 and OD-19) revealed a total of 13 bands among the accessions ranging in the size from 21.5 to 92.0 kd. Six citral rich accessions exhibits very similar seed protein profile with 10 to 11 protein band each. However, the geraniol rich chemotype RLJ-TC-8 exhibit different profile with only six high molecular proteins. Four seed proteins with molecular weight 92.0, 86.0, 80.4 and 61.6 kd were consistently found in all the chemotypes irrespective of citral or geraniol rich and can be considered as marker for the species. Although esterase isozymes exhibited low polymorphism, yet close similarity of isozyme and seed protein profile can be considered as evidence of genetic homogeneity among the accession of lemongrass.
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