Effect of peucedanin and bergapten (5-MOP), furanocoumarines isolated from Peucedanum tauricum Bieb.(Apiaceae) fruits, on apoptosis induction and heat-shock proteins expression in HeLa cells
In the research of bergapten (5-MOP) and peucedanin, furanocoumarines isolated from Peucedanum tauricum Bieb.(Apiaceae) fruits, on apoptosis, necrosis and heat-shock proteins Hsp 27 and Hsp 72 expression in human carcinoma cells HeLa (ECACC cells No 85060701) were preliminary studied. The purity of analyzed compounds was confirmed by RP HPLC, EI-MS and 1D, 2D NMR. For apoptosis and necrosis detection the mixture of Hoechst 33342 and propidium iodide (PI) was used. Early apoptosis was detected using Annexin V Test.