Application of green-extraction technique to evaluate of antioxidative capacity of wild population of fireweed (Epilobium angustifolium)

ANNA NOWAK, ADAM KLIMOWICZ, WIKTORIA DUCHNIK, ŁUKASZ KUCHARSKI, KATARZYNA FLORKOWSKA, ANNA MUZYKIEWICZ, DARIA WIRA, JOANNA ZIELONKA-BRZEZICKA, ANITA SIEDŁOWSKA, KINGA NADARZEWSKA

Abstract

Introduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields. Objective: The aim of the study was to evaluate the antioxidant activity of extracts from fresh fireweed, harvested at three ripening stages. Methods: Analysis of antioxidative activity was carried out by DPPH, ABTS and FRAP methods. Total polyphenol and total flavonoid content were also determined. Plant material was extracted using ultrasound- assisted green extraction technique with methanol, ethanol and isopropanol at different concentrations and water. Results: The highest antioxidant activity evaluated by DPPH, ABTS and FRAP was found for the extracts prepared in 70% ethanol. The highest content of total polyphenols were observed in extracts in 70% ethanol, whereas the highest content of flavonoids extracts in undiluted methanol. Conclusion: Epilobium angustifolium harvested at fruit ripening stage seems to be a valuable source of antioxidants.
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