Chlorogenic acid in in vitro cultures of Eleutherococcus senticosus



The establishment of callus and cell suspension cultures of Eleutherococcus senticosus was reported. The four callus lines were obtained from stolon and bud explants from a fieldgrown plant. They were cultured on Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) agar media, supplemented with 30 g/l sucrose, 2,4-dichlorophenoxyacetic acid (2,4-D) (1 mg/l), á-naphtaleneacetic acid (NAA; 1 mg/l) and 6-benzylaminopurine (BAP; 0.5 mg/ l). The cell suspension culture was also maintained in SH liquid medium with the same growth regulators and sucrose (50 g/l). HP LC analysis showed that the cultures produced chlorogenic acid. The highest accumulation of the compound was detected in the cell culture (29.7 mg/g d.w.); it was 15 times the average level reported for starting callus, several times as much as roots, and far more than leaves of intact plants.
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