Chlorogenic acid in in vitro cultures of Eleutherococcus senticosus

K.FLORYANOWICZ-CZEKALSKA, A.PODSĘDEK, H.WYSOKIŃSKA

Abstract

The establishment of callus and cell suspension cultures of Eleutherococcus senticosus was reported. The four callus lines were obtained from stolon and bud explants from a fieldgrown plant. They were cultured on Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) agar media, supplemented with 30 g/l sucrose, 2,4-dichlorophenoxyacetic acid (2,4-D) (1 mg/l), á-naphtaleneacetic acid (NAA; 1 mg/l) and 6-benzylaminopurine (BAP; 0.5 mg/ l). The cell suspension culture was also maintained in SH liquid medium with the same growth regulators and sucrose (50 g/l). HP LC analysis showed that the cultures produced chlorogenic acid. The highest accumulation of the compound was detected in the cell culture (29.7 mg/g d.w.); it was 15 times the average level reported for starting callus, several times as much as roots, and far more than leaves of intact plants.
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