The estimation of antifungal activity of essential oil and hydrosol obtained from wrinkled-leaf mint (Mentha crispa L.)
The effects of essential oil and hydrosol extracted from dried aerial parts of wrinkled-leaf mint (Mentha crispa L.), were determined against Aspergillus fumigatus, Aspergillus parasiticus, Botrytis cinerea, Cladosporium herbarum, Fusarium oxysporum and Penicillium cyclopium growth. The influence of oil was estimated by disc diffusion agar method and expressed (in mm) as inhibition growth zones of tested strains. Furthermore, the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of oil were determined by microdilution method in liquid medium. Chromatography analysis showed that the main components of oil were: piperitenone (29.9%), pulegone (13.9%) and 1,8-cineole (10.5%). The oil proved the highest inhibitory action against B. cinerea (MFC≤0.32 μl·ml-1, inhibition zone 50.0 mm) and C. herbarum (MFC=0.63 μl·ml-1, inhibition zone 59.3mm), and the least – against A. parasiticus and F. oxysporum, (inhibition zones respectively 7.0 and 12.7 mm, MIC=5.0 μl·ml-1, and MFC=10.0 μl·ml-1). Activity of hydrosol was expressed as a percent of inhibition growth of fungi which was calculated on the ground of diameters of colonies growing on agar medium with addition of 10 and 15% hydrosol and diameters of colonies on control medium. Data showed that antifungal activity of hydrosol was weak. It in higher amounts inhibited the growth of A. fumigatus (by 8.7 and 15.9%, respectively, at a concentration in medium of 10 and 15%) and stimulated growth of B. cinerea and P. cyclopium.