Determination of chlorogenic and gallic acids by UPLC-MS/MS
Abstract
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids:
chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola:
R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts
were prepared. In order to identify analysed polyphenolic compounds ultra performance
liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic
acid is commonly found in the roots of these plants. Aqueous extract in both species
is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method
for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic
method developed by our team is more precise then previously published.