Novel method of detection of phenylpropanoids of Rhodiola roots species
Abstract
The aim of the study was the identification and quantitative analysis of phenylpropanoid
compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined
in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres
and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography
- tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results
showed differences in the quantitative and qualitative assessments of these two species. In
the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the
most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to
use this analytical method for determination of phenylpropanoids in the accordance with
the requirements of ICH.