Novel method of detection of phenylpropanoids of Rhodiola roots species

AGNIESZKA GRYSZCZYŃSKA, BOGNA OPALA, ANNA KRAJEWSKA-PATAN, ZDZISŁAW ŁOWICKI, WALDEMAR BUCHWALD, SEBASTIAN MIELCAREK, ANNA BOGACZ, MONIKA KARASIEWICZ, DARIUSZ BOROŃ, BOGUSŁAW CZERNY, PRZEMYSŁAW M. MROZIKIEWICZ

Abstract

The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.
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