The antimicrobial activity of ethanolic extracts from leaves and roots of three Eryngium L. genera (E. planum, E. campestre, E. maritimum) native to Poland was tested by the method of series dilutions against different Gram-positive bacteria (two strains) and fungi (five species). The extracts were analyzed by TLC method to confirm phenolic acids, triterpenoid saponins, flavonoids and acetylenes presence. The antimicrobial activity of extracts compared with the reference substance were expressed by Minimal Inhibitory Concentration (MI C). The results have shown that the ethanolic extracts inhibit the growth of Staphylococcus aureus and all tested fungi.